Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
HAP1 (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 13585-1-AP incubated at 1:2000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
WB result of PRDX6 antibody (13585-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells.
Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 13585-1-AP coupled to protein A- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP Result of anti-PRDX6 (IP:13585-1-AP, 3ug; Detection:13585-1-AP 1:500) with HeLa cells lysate 2800ug.
HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 13585-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens).