PARP1 VHH, recombinant binding protein
Description
Alpaca anti-PARP1 VHH, purified protein, unconjugated
Synonyms
PARP1-Binding-Protein, PARP1-Nanobody, and PARP1-single domain antibody (sdAb)
Specificity
Human PARP1
Epitope: within DNA binding domain of PARP1
The anti-PARP1 VHH does not bind to human PARP2, 3, or 9
Applications
Coupling of dyes, biotin, etc. via NHS Ester reaction
| Product | Size | Code | Price | Buy |
|---|---|---|---|---|
| Product PARP1 VHH, recombinant binding protein | Size 250 µL | Code xt-250 | Price Please inquire |
Specificity
PARP1, human
Conjugation:
Unconjugated
Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
Biophysical properties
Molecular weight MW: 14.36 kDa
Molecular extinction coefficient ε: 17,670 L·Mol−1·cm−1
Isoelectrical point pI: 9.2 (theoretical)
Form
Purified recombinant antibody fragment, monoclonal
Protein concentration
1 mg/mL
Storage buffer
25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.
Do not freeze.
Are the nanobodies monoclonal or polyclonal?
The nanobodies are recombinant/monoclonal.
What are the biophysical parameters of the Nanobodies?
| Name | MW (kDa) | Molar Extinction Coefficient (M-1 cm-1) | Theoretical pI |
|---|---|---|---|
| GFP VHH | 13.9 | 27.055 | 9.4 |
| GST VHH | 14.8 | 28.545 | 8.2 |
| Halo VHH | 14.8 | 23.045 | 9.4 |
| Histon VHH | 14.4 | 25.565 | 10.2 |
| MBP VHH | 14.1 | 27.055 | 10.0 |
| Mdm4/Hdmx VHH | 13.6 | 14.565 | 10.1 |
| MK2 VHH | 14.4 | 32.555 | 9.7 |
mNeonGreen VHH | 14.3 | 21.680 | 8.0 |
| Myc VHH | 13.5 | 23.045 | 9.6 |
| p53 C-term VHH | 14.8 | 34.170 | 9.2 |
| p53 N-term VHH | 13.0 | 20.065 | 9.7 |
| PARP1 VHH | 14.4 | 17.670 | 9.2 |
| RFP VHH | 14.9 | 30.035 | 9.3 |
| SNAP/CLIP-tag VHH | 13.1 | 24.075 | 9.8 |
| Spot VHH (bivalent) | 30.3 | 46.340 | 5.3 |
| TurboGFP VHH | 13.6 | 18.575 | 9.5 |
| Vimentin VHH | 26.7 | 48.150 | 10.0 |
Do the nanobodies have any tags?
Yes, the nanobodies have a C-terminal His6-tag.
Extraordinary stable & reliable binding
- Applications validated and characterized
Related products
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PARP1-Chromobody
Only for research applications, not for diagnostic or therapeutic use!