Myc-Trap® Agarose is an affinity reagent for immunoprecipitation and purification of Myc-tagged proteins.
It consists of a Myc Nanobody/ VHH coupled to agarose beads.
Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein.
Endogenous c-myc is NOT bound.
ChIP/ RIP analysis
On-bead enzyme assays
|Product Myc-Trap Agarose||Size 250 µL (10 reactions)||Code yta-10||Price $ 275||Buy +|
|Product Myc-Trap Agarose||Size 500 µL (20 reactions)||Code yta-20||Price $ 475||Buy +|
|Product Myc-Trap Agarose||Size 2.5 mL (100 reactions)||Code yta-100||Price $ 2050||Buy +|
|Product Myc-Trap Agarose||Size 5 mL (200 reactions)||Code yta-200||Price Please inquire|
|Product Myc-Trap Agarose||Size 10 mL (400 reactions)||Code yta-400||Price Please inquire|
|Product Myc-Trap Agarose Kit||Size 500 µL (20 reactions)||Code ytak-20||Price $ 595||Buy +|
|Product Myc Peptide||Size 1 mg||Code yp-1||Price $ 85||Buy +|
|Product Myc Peptide||Size 10 mg||Code yp-10||Price $ 640||Buy +|
|Product 2x Myc Peptide||Size 1 mg||Code 2yp-1||Price $ 105||Buy +|
|Product Binding Control Agarose Beads||Size 500 µL (20 reactions)||Code bab-20||Price $ 75||Buy +|
|Product Spin Columns||Size 10 units||Code sct-10||Price $ 35||Buy +|
|Product Spin Columns||Size 20 units||Code sct-20||Price $ 60||Buy +|
|Product Spin Columns||Size 50 units||Code sct-50||Price $ 130||Buy +|
|Product Myc VHH, recombinant binding protein||Size 250 µL||Code yt-250||Price $ 275||Buy +|
1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Wash buffer compatibility
2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Myc-tag sequence motif EQKLISEEDL
10 µL slurry bind about 7 µg of recombinant Myc-tagged MBP
Coupled Nanobody/ VHH
Recombinant, monoclonal anti-Myc-tag single domain antibody (sdAb) fragment
Bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Storage buffer: 20 % EtOH
- 1x Myc peptide, 2x Myc peptide. For details see below.
- SDS sample buffer
- 0.2 M glycine pH 2.5
Please consider whether elution is really needed. We recommend on-bead assays like on-bead digestion for MS analysis.
Compatibility with mass spectrometry
The Myc-Trap is optimized for on-bead digestion. Complete tryptic digest results in 6-7 peptides.
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.
Does the Myc-Trap bind endogenous c-Myc protein?
We could not detect binding of endogenous c-Myc protein to the Myc-Trap. Some epitope residues that have shown to be crucial for binding to the Myc-Trap are buried in the three-dimensional structure of the c-Myc protein. Hence, under native conditions, c-Myc protein is not a suitable binding partner for the Myc-Trap.
How can I gently elute native Myc-tagged protein from the Myc-Trap in its native state?
You can elute your Myc-fusion competitively with 1x or 2x Myc peptide. Alternatively, you can use 8 M Urea or 0.2 M glycine pH 2.5 at room temperature.
Should I use 1x Myc- or 2x Myc-peptide for elution of the Myc-Trap?
It is likely that the Myc-Trap binds several motifs of a double Myc-tag. Hence, elution of a Myc-tagged fusion protein is more efficient with the 2x Myc-peptide. In addition, the term "Myc-tag" can refer to a single or double Myc-tag. To be on the safe side, we generally recommend elution with the 2x Myc-peptide.
Should I use an N-terminal or C-terminal fusion tag?
Both the N-terminal or C-terminal fusion tag work well with the traps.
How can I avoid unspecific protein interactions binding to the trap?
How can I elute bound proteins from a trap in their native state?
You can elute your fusion protein of interest with 0.2 M glycine pH 2.5 at room temperature. Pipette the beads up and down for 60-120 seconds and repeat this step. Ensure to neutralize your supernatant immediately afterwards by adding 1 M Tris base pH 10.4.
How many mammalian cells are required for an immunoprecipitation reaction?
For one immunoprecipitation reaction, we recommend using ~10^6 - 10^7 mammalian cells. The yield is also dependent on the expression level of your protein of interest and the interaction partners.
How much cell extract should I use for an immunoprecipitation reaction?
For other type of cells than mammalian cells, we recommend using 0.5 - 1.0 mg of cell extract.
Do I need to elute bound proteins from the beads for mass spectrometry analysis?
Do I need to elute my protein of interest from the beads for enzymatic assays?
What is the amount of trap slurry I need for one immunoprecipitation reaction?
25 µL slurry are sufficient for one pull-down reaction as the affinity of the traps is very high.
- Efficient and fast pulldown of Myc-tagged proteins
- No heavy & light antibody chains in downstream applications
- One step immunoprecipitation
- Easy elution of native proteins
1x Myc-Tag or 2x Myc-Tag: Binding and Elution
Both, 1x Myc- and 2x Myc-tagged Protein of Interest (POI) can be used for all kind of IPs, Co-IPs, and affinity purification. To further optimize your experiment:
- Use 1x Myc-tagged POI if you like to effectively or gently elute your protein of interest from Myc-Trap in native conditions.
- Use 2x Myc-tagged POI if you like to effectively pull-down/bind low expressed/abundant proteins. For downstream assays we recommend on-bead applications like on-bead digestion and on-bead assays.
|1x Myc-Tag||2x and 3x Myc-Tag|
|1x Myc-peptide (40 µM)||++||o|
|2x Myc-peptide (40 µM)||+++||+|
|Urea (8 M)||+++||+|
|Glycine (0.2 M) pH 2.5||+||+|
Elution of 1x, 2x and 3x Myc-tagged protein from Myc-Trap.
Myc-Trap Consideration Aspects
1x Myc-fusion protein
2x and 3x Myc-fusion protein
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For details, see white paper
Myc-Trap Agarose Kit
The Myc-Trap Agarose is also available in a kit, including:
- Myc-Trap Agarose
- Lysis*, wash and elution buffers
*The lysis buffer provided is suitable for mammalian cells. For other cell types use another suitable lysis buffer.
- Easy and convenient handling
- Rapid washing and clean elution of bound proteins
- Simplify the pulldown of your protein
- Spin columns for Myc-Trap Agarose
Binding Control for Agarose Beads
- Control for unspecific binding of proteins, DNA, etc. to beads
- Pre-clearing of cell lysate
- Binding control for Myc-Trap Agarose
The Myc peptides may be used to elute native and functional Myc-tagged fusion proteins bound to Myc-Trap® or anti-Myc antibodies.
- Easy elution of Myc-tagged fusion proteins
Only for research applications, not for diagnostic or therapeutic use!