Chromobodies®
Intracellular Nanobodies for live-cell analysis in real-time
Chromobodies are fluorescent nanoprobes for real-time and live-cell imaging of intracellular endogenous proteins. A Chromobody is a small intracellular functional antibody. It consists of a VHH / Nanobody that is genetically fused to a fluorescent protein.
Unlike conventional antibodies, Chromobodies visualize endogenous cellular structures and processes in live cells and real-time. Chromobody probes label non-invasively their targets without interfering with target function. This makes them perfect nanoprobes for cellular research and high content analysis.
Chromobodies are supplied as DNA plasmids. Upon transfection, they allow for dynamic live-cell analysis of endogenous proteins:
- Fast no-wash assay
- No overexpression of the protein of interest
- No cytotoxicity and artificial effects
Applications:
- Live-cell imaging of endogenous proteins
- High content analysis of cells, tissues, whole organs (sections), and organisms (sections)
- Investigation of small molecules in secondary screens – either as biomarkers or controls
Scheme of a cell expressing Chromobodies upon transfection with the Chromobody DNA plasmid. The Chromobody plasmid codes for a VHH/ Nanobody that is genetically fused to GFP. After transfection with the Chromobody plasmid, the Chromobody is expressed within the cell: The Nanobody part binds to the epitope and the GFP can be detected with a microscope.
| Product | Formats (Product citations in parentheses) |
|---|---|
| Actin-Chromobody® | TagGFP2 (15), TagRFP (5) |
| Nuclear Actin-Chromobody® | TagGFP2 |
| Cell Cycle-Chromobody® | TagRFP (11) |
| Dnmt1-Chromobody® | TagGFP2, TagRFP (1) |
| Histone-Chromobody® | eGFP (1) |
| Lamin-Chromobody® | TagGFP2 (5) |
| PARP1-Chromobody® | TagGFP2, TagRFP (2) |
| Vimentin-Chromobody® | TagGFP (2) |